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KMID : 0545120060160101544
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Journal of Microbiology and Biotechnology
2006 Volume.16 No. 10 p.1544 ~ p.1553
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Identification of Antiangiogenic and Anti-Cell Adhesion Materials from Halophilic Enterobacteria of the Trachurus japonicus
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Lim Jong-Kwon
Seo Hyo-Jin
Kim Eun-Ok
Meidany Mohsen
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Abstract
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The halophilic enterobacteria, Enterobacteria cancerogenus, was isolated from the intestines of the fusiform fish (Trachurus japonicus) to yield a protein-like material termed PLM-f74. PLM-f74 was characterized by strong inhibition ratios to angiogenesis (82.8% at the concentration of ) and elevated antioxidative capacities with low toxicity. The PLM-f74 is a glycoprotein comprised of saccharides and amino acids. PLM-f74 inhibited cell adhesion that non-activated U937 monocytic cell adhesion to HUVECs activated with by 78.0%, and the adherence of U937 cells treated with the PLM-f74 and stimulated with to unstimulated HUVECs decreased by 102%. When both cell types were pretreated with PLM-f74, the adhesion of U937 cells to -stimulated HUVECs was completely suppressed by 121% at a concentration of . PLM-f74 blocked signal pathways from VEGFR2, PI3K, -catenin, and VE-cadherin to NF-kB, based on western bolt analysis. It also inhibited IL-l-stimulated HUVEC expression of the adhesion molecules, ICAM-l by 40%, VCAM-l by 60%, and E-selectin by 70% at the same concentration noted above. New anti-angiogenic and anti-cell adhesion materials showing elevated antioxidative capacities, and non-toxicity may be expected from these results.
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KEYWORD
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Antiangiogenesis, antioxidative capacity, cytotoxicity, fusiform fish, cell adhesion, protein-like material (PLM)
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